Single Sequence tutorial

Explore how sequence-encoded biophysical properties vary across a protein.

The selector below chooses a protein from the original TIM-barrel example used by b2btools. It includes natural TIM-barrel proteins, the de novo designed sTIM-11 protein, and OctaV1, which did not fold into the intended barrel.

The plot shows predictions against residue position. Hover over a residue for its values and click prediction names in the legend to show or hide them. These values describe properties the sequence can encode; they do not guarantee a particular folded structure.

Bs

Biophysical features overview

Reading the predictions

  • DynaMine backbone dynamics: values below 0.69 suggest flexibility; values above 0.80 suggest rigidity.
  • Conformational propensities: higher helix, sheet, coil, or ppII values indicate a higher local propensity.
  • EFoldMine: values above 0.169 identify residues likely to begin folding through local interactions.
  • DisoMine: values above 0.5 indicate likely disorder.

Continue with the MSA tutorial to compare a sequence with its homologues, or submit your own sequence from the single-sequence predictors.