Bio2Byte tools web interface

About this page

Our web interface gives integrated access to individual predictors, and includes a novel feature to explore prediction-based 'biophysical variation' of proteins from a multiple sequence alignment (the complete list of the our software tools is available here).

STEP 1: Enter your input sequences

Currently, the system takes FASTA files, a range of multiple sequence alignment (MSA) formats, and the NEF and NMR-STAR formats for NMR chemical shift data (ShiftCrypt).

N:

CA:

CB:

C:

H:

Online available tools

Depending on the format of your input data, in the second step of this web interface, you will be able to select predictors from these list:

DynaMine
  •  Prediction of protein backbone dynamics from sequence only
  •  Up to 50 sequences
  •  Min. 5 residues per sequence
  •  Supported format(s): FASTA
  •  Predictions: backbone, sidechain, sheet, helix, coil, polyproline II
EFoldMine
  •  Prediction of protein early folding regions from sequence only
  •  Up to 50 sequences
  •  Min. 5 residues per sequence
  •  Supported format(s): FASTA
  •  Predictions: earlyFolding
DisoMine
  •  Prediction of protein disorder from sequence only
  •  Up to 50 sequences
  •  Min. 5 residues per sequence
  •  Supported format(s): FASTA
  •  Predictions: disoMine
AgMata
  •  Prediction of protein regions that are likely to cause beta-aggregation
  •  Up to 10 sequences
  •  Min. 5 residues per sequence
  •  Supported format(s): FASTA
  •  Predictions: agmata
PSP (Phase Separating Protein)
  •  Predict whether a protein is likely to phase-separate with a particular mechanism involving RNA interacts (FUS-like proteins)
  •  Up to 20 sequences
  •  Min. 20, max. 3000 residues per sequence
  •  Supported format(s): FASTA
  •  Predictions: PSPer score, Complexity, Tyr, Arg, RRM, Disorder
ShiftCrypt
  •  Enable the compression of chemical shift information for proteins into single per-residue values that reflect the in-solution biophysical properties of those residues
  •  Up to 50 sequences
  •  Min. 5 residues per sequence
  •  Supported format(s): NEF, NMR-Star
  •  Predictions: ShiftCrypt score
MSA Tools
  •  Prediction of biophysical parameters (from DynaMine, DisoMine, and EFoldMine) within the multiple sequence alignment (MSA).
  •  Up to 200 aligned sequences
  •  Min. 5 residues per sequence
  •  Supported format(s): CLUSTAL, FASTA, BaliBase, PSI, A3M, Blast, PHYLIP, STOCKHOLM
  •  Predictions: backbone, sidechain, sheet, helix, coil, polyproline II, earlyFolding, disoMine

Alternative methods

This tools are also available directly through an REST API, find the guidelines and examples on the API documentation section. In addition, we provide a Python Package with all our tools (Single Sequence, MSA, and general tools such as parsing and plotting) to enable you to integrate these predictors in your pipelines. For more information, please visit our site on the Python Package Index: b2BTools package.

Further information

Please read the usage guidelines provided on the tutorial section. If you have any feedback or found an issue, please contact us using the feedback from. Read our Terms of use if you are concerned with your privacy and how we handle personal information.