Entry STF0052
Nanobody from Lama glama
Protein information
| Name of the protein: | H14 protein |
| Organism: | Lama glama |
| Number of residues: | 117 |
| Related UniProt entry: | A2KD53 (Fragment: 2 - 117) |
| Related PDB entry: | 1HCV |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 4.6; 27.0 Celsius; Probes: 54
Related publication:
PMID 11141058
Experiment details: "For the hydrogen-deuterium exchange experiments, lyophilized, uniformly 15N-labeled VHH-H14 was dissolved in D2O buffer [10 mM deuterated sodium acetate (99.5% D), 100 mM NaCl, and 0.02% sodium azide (pH 4.6)] to a concentration of 0.5 mM. The pH meter readings were corrected for the isotope effect."
Protection threshold: log(P) > 4
Sequence:
DVQLQESGGGLVQAGGSLRLSCAASGRTGSTYDMGWFRQAPGKERESVAAINWDSARTYYASSVRGRFTISRDNAKKTVYLQMNSLKPEDTAVYTCGAGEGGTWDSWGQGTQVTVSS
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STRONG residues
18: L;
20: L;
34: M;
35: G;
36: W;
37: F;
38: R;
49: A;
51: I;
69: T;
71: S;
80: Y;
81: L;
82: Q;
83: M;
87: K;
90: D;
91: T;
92: A;
94: Y;
97: G;
109: Q;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 4.6; 27.0 Celsius; Probes: 54
Related publication:
PMID 11141058
Experiment details: "For the hydrogen-deuterium exchange experiments, lyophilized, uniformly 15N-labeled VHH-H14 was dissolved in D2O buffer [10 mM deuterated sodium acetate (99.5% D), 100 mM NaCl, and 0.02% sodium azide (pH 4.6)] to a concentration of 0.5 mM. The pH meter readings were corrected for the isotope effect."
Protection threshold: 2 < log(P) < 4
Sequence:
DVQLQESGGGLVQAGGSLRLSCAASGRTGSTYDMGWFRQAPGKERESVAAINWDSARTYYASSVRGRFTISRDNAKKTVYLQMNSLKPEDTAVYTCGAGEGGTWDSWGQGTQVTVSS
CLICK TO DOWNLOAD SEQUENCE IN FASTA
MEDIUM residues
12: V;
14: A;
16: G;
17: S;
21: S;
23: A;
46: E;
48: V;
50: A;
52: N;
61: A;
68: F;
84: N;
93: V;
95: T;
99: G;
106: S;
108: G;
113: V;
114: T;
115: V;
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