Entry STF0049
Alpha-lactalbumin (human)
Protein information
Name of the protein: | Alpha-lactalbumin |
Organism: | Homo sapiens |
Number of residues: | 123 |
Related UniProt entry: | P00709 (Fragment: 21 - 142) |
Related PDB entry: | 3B0O |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.3; 15.0 Celsius; Probes: 45
Related publication:
PMID 7473740
Experiment details: "Hydrogen-deuterium exchange was initiated by dissolving lyophilized samples either in D2O, pH* 2 (molten globule) or native exchange buffer (100 mM d4-imidazole, 3 mM CaCl2 with a final pH* of 6.3). The protein concentration was 80 mM for exchange in the molten globule and 800 mM for exchange in the native state. For the molten globule, exchange was quenched by freezing in liquid nitrogen and subsequent lyophilization at regular intervals from ten seconds to nine days. Dry samples were stored at −80 °C and dissolved in native exchange buffer for analysis."
Protection threshold: k(int)/k(ex) > 100000
Sequence:
MQFTKCELSQLLKDIDGYGGIALPELICTMFHTSGYDTQAIVENNESTEYGLFQISNKLWCKSSQVPQSRNICDISCDKFLDDDITDDIMCAKKILDIKGIDYWLAHKALCTEKLEQWLCEKL
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STRONG residues
33: T;
61: C;
73: C;
77: C;
80: F;
91: C;
92: A;
93: K;
94: K;
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STRONG
Method: Native exchange in partially folded state by NMR
Conditions: pH 2.0; 5.0 Celsius; Probes: 34
Related publication:
PMID 7473740
Experiment details: "Hydrogen-deuterium exchange was initiated by dissolving lyophilized samples either in D2O, pH* 2 (molten globule) or native exchange buffer (100 mM d4-imidazole, 3 mM CaCl2 with a final pH* of 6.3). The protein concentration was 80 mM for exchange in the molten globule and 800 mM for exchange in the native state. For the molten globule, exchange was quenched by freezing in liquid nitrogen and subsequent lyophilization at regular intervals from ten seconds to nine days. Dry samples were stored at −80 °C and dissolved in native exchange buffer for analysis."
Protection threshold: k(int)/k(ex) > 100
Sequence:
MQFTKCELSQLLKDIDGYGGIALPELICTMFHTSGYDTQAIVENNESTEYGLFQISNKLWCKSSQVPQSRNICDISCDKFLDDDITDDIMCAKKILDIKGIDYWLAHKALCTEKLEQWLCEKL
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STRONG residues
26: L;
27: I;
30: M;
33: T;
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