Entry STF0048
Alpha-lactalbumin (guinea pig)
Protein information
| Name of the protein: | Alpha-lactalbumin |
| Organism: | Cavia porcellus |
| Number of residues: | 123 |
| Related UniProt entry: | P00713 (Fragment: 20 - 142) |
| Related PDB entry: | 1HFX |
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Experiment sets
STRONG
Method: Native exchange in partially folded state by NMR
Conditions: pH 2.0; 25.0 Celsius; Probes: 21
Related publication:
PMID 8504087
Experiment details: "The hydrogen exchange kinetics of individual amide protons in the A-state at pH 2.0 were measured by pH-jump experiments which were performed in three steps: (a) Fully protonated GPLA was dissolved in H2O, the pH was lowered to 2.0, and then the protein was lyophilized. (b) The protein was then dissolved in D2O, giving a solution of the A-state without the need for further pH adjustment. (c) After various lengths of time, aliquots of the protein were restored to the native state by carefully raising the pH to 5.5. The indole hydrogen of W26 has the highest protection factor of > 920."
Protection threshold: P > 70
Sequence:
KQLTKCALSHELNDLAGYRDITLPEWLCIIFHISGYDTQAIVKNSDHKEYGLFQINDKDFCESSTTVQSRNICDISCDKLLDDDLTDDIMCVKKILDIKGIDYWLAHKPLCSDKLEQWYCEAQ
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STRONG residues
26: W;
28: C;
29: I;
30: I;
31: F;
95: I;
96: L;
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