Alpha-lactalbumin (guinea pig)
|Name of the protein:||Alpha-lactalbumin|
|Number of residues:||123|
|Related UniProt entry:||P00713 (Fragment: 20 - 142)|
|Related PDB entry:||1HFX|
Visualize the data
Click here or on the image on the right to visualize the residues using JSmol. Warning: JSmol is known to load slowly on certain browsers, depending on the size of the macromolecule. The applet is optimized for Chrome, other browsers have limited support.
Method: Native exchange in partially folded state by NMR
Conditions: pH 2.0; 25.0 Celsius; Probes: 21
Experiment details: "The hydrogen exchange kinetics of individual amide protons in the A-state at pH 2.0 were measured by pH-jump experiments which were performed in three steps: (a) Fully protonated GPLA was dissolved in H2O, the pH was lowered to 2.0, and then the protein was lyophilized. (b) The protein was then dissolved in D2O, giving a solution of the A-state without the need for further pH adjustment. (c) After various lengths of time, aliquots of the protein were restored to the native state by carefully raising the pH to 5.5. The indole hydrogen of W26 has the highest protection factor of > 920."
Protection threshold: P > 70
CLICK TO DOWNLOAD SEQUENCE IN FASTA
CLICK TO DOWNLOAD LIST OF RESIDUES