Entry STF0043
Interleukin-1b subunit
Protein information
| Name of the protein: | Interleukin-1 beta |
| Organism: | Homo sapiens |
| Number of residues: | 153 |
| Related UniProt entry: | P01584 (Fragment: 117 - 269) |
| Related PDB entry: | 1I1B |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.1; 36.0 Celsius; Probes: 73
Related publication:
PMID 2372550
Experiment details: "We have made accurate measurements of the exchange rates of 73 slowly amide NH groups in IL-1b by following the intensities of the 15N-'H correlation peaks in a series of double INEPT "Overbodenhausen" experiments recorded after dissolving freeze-dried protein into D2O."
Protection threshold: exchange half-lives longer than 20 days
Sequence:
APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
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STRONG residues
68: Y;
69: L;
72: V;
81: Q;
83: E;
100: V;
101: F;
113: E;
115: A;
117: F;
121: Y;
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EARLY
Method: Quenched-flow HDX NMR
Conditions: pH 5.0; 4.0 Celsius; Probes: 47
Related publication:
PMID 8493553
Experiment details: "Fully unfolded protein in D2O at pH 5.0 is allowed to fold for a length of time T, after which it is subjected to a 16-ms exchange pulse in H2O at pH 9.3, followed immediately by a quench pulse at pH 5.0."
Protection threshold: amide protection half-lives between 0.7 and 1.5 s
Sequence:
APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
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EARLY residues
42: F;
68: Y;
69: L;
70: S;
72: V;
79: T;
81: Q;
83: E;
99: F;
100: V;
101: F;
102: N;
103: K;
104: I;
111: E;
113: E;
114: S;
117: F;
120: W;
121: Y;
123: S;
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INTERMEDIATE
Method: Quenched-flow HDX NMR
Conditions: pH 5.0; 4.0 Celsius; Probes: 47
Related publication:
PMID 8493553
Experiment details: "Fully unfolded protein in D2O at pH 5.0 is allowed to fold for a length of time T, after which it is subjected to a 16-ms exchange pulse in H2O at pH 9.3, followed immediately by a quench pulse at pH 5.0."
Protection threshold: amide protection half-lives between 15 and 25 s
Sequence:
APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
CLICK TO DOWNLOAD SEQUENCE IN FASTA
INTERMEDIATE residues
43: S;
58: V;
71: C;
115: A;
132: V;
146: F;
147: T;
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