Entry STF0039
Chymotrypsin Inhibitor 2
Protein information
| Name of the protein: | Subtilisin-chymotrypsin inhibitor-2A |
| Organism: | Hordeum vulgare |
| Number of residues: | 64 |
| Related UniProt entry: | P01053 (Fragment: 19 - 84) |
| Related PDB entry: | 3CI2 |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 5.3; 33.0 Celsius; Probes: 34
Related publication:
PMID 9231903
Experiment details: "Experiments were perfomed at 33 °C in 50 mM sodium acetate buffer (pH 5.3). The total salt concentration was maintained at 2.5 M throughout the range of GdmCl concentrations by addition of NaCl. Values of m and ΔGapp,0ex were calculated by fitting the exchange data at different [GdmCl] to the equation. Fitting was performed only when k(ex) could be measured for more than six [GdmCl]."
Protection threshold: ΔΔG(ex) = ΔΔG(global unfolding)
Sequence:
HNLKTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVGTIVTMEYRIDRVRLFVDKLDNIAQVPRVG
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STRONG residues
13: K;
22: I;
23: L;
32: I;
34: L;
49: V;
51: L;
52: F;
53: V;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 5.3; 33.0 Celsius; Probes: 34
Related publication:
PMID 9231903
Experiment details: "Experiments were perfomed at 33 °C in 50 mM sodium acetate buffer (pH 5.3). The total salt concentration was maintained at 2.5 M throughout the range of GdmCl concentrations by addition of NaCl. Values of m and ΔGapp,0ex were calculated by fitting the exchange data at different [GdmCl] to the equation. Fitting was performed only when k(ex) could be measured for more than six [GdmCl]."
Protection threshold: m > 2
Sequence:
HNLKTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVGTIVTMEYRIDRVRLFVDKLDNIAQVPRVG
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MEDIUM residues
7: W;
10: L;
11: V;
20: K;
21: V;
24: Q;
34: L;
48: R;
50: R;
54: D;
59: I;
60: A;
61: Q;
64: R;
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