Entry STF0038
Bovine pancreatic trypsin inhibitor
Protein information
Name of the protein: | Pancreatic trypsin inhibitor |
Organism: | Bos taurus |
Number of residues: | 58 |
Related UniProt entry: | P00974 (Fragment: 36 - 93) |
Related PDB entry: | 5PTI |
Visualize the data
Click here or on the image on the right to visualize the residues using JSmol. Warning: JSmol is known to load slowly on certain browsers, depending on the size of the macromolecule. The applet is optimized for Chrome, other browsers have limited support.
Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 3.5; None Celsius; Probes: 13
Related publication:
PMID 7248461
Experiment details: "Exchange rates for 13 individual, assigned peptide amide protons have been measured for BPTI as a function of pH and temperature. The rates of the slowest exchanging protons group together at pH ~4; these are also the most down-shifted of the resonances in the PMR spectra. Their complete pH-rate profiles from 20-680 °C have been reported."
Protection threshold: slowest exchanging protons
Sequence:
RPDFCLEPPYTGPCKARIIRYFYNAKAGLCQTFVYGGCRAKRNNFKSAEDCMRTCGGA
CLICK TO DOWNLOAD SEQUENCE IN FASTA
STRONG residues
20: R;
21: Y;
22: F;
23: Y;
31: Q;
33: F;
45: F;
CLICK TO DOWNLOAD LIST OF RESIDUES
EARLY
Method: HDX-folding competition by NMR
Conditions: pH 4.0-7.5; None Celsius; Probes: 8
Related publication:
PMID 2835760
Experiment details: "An H2O solution of the fully denatured protein at in 40% (vh) n-propanol at pH 2.0 was mixed with 2 volumes of 0.2 M phosphate buffer in D2O at a range of pD values between about 4.5 and 7.5. This produces conditions at pH values above 4.0 in a 1:2 H2O/D2O mixture containing 13% n-propanol. The initial protein concentration in the H2O solution was 3 mM. pH was measured at 70°C in aliquots of the refolding mixture and is reported without correction for isotope effects or the presence of n-propanol. Syringes, mixer, and reaction tubing were thermostated at 70 °C. For quenching, the mixture was injected through a nozzle into isopentane at -70°C or ice water. The nozzle was placed a few millimeters above the liquid. The reaction time was determined from the calibrated flow rate and the precisely measured reaction volume, i.e., the volume between mixer and quencher. The shortest reaction time achieved was 30 ms. Longer times were set by reduction of the flow rate and/or increased length of the reaction tubing (thermostated at 70°C)."
Protection threshold: -
Sequence:
RPDFCLEPPYTGPCKARIIRYFYNAKAGLCQTFVYGGCRAKRNNFKSAEDCMRTCGGA
CLICK TO DOWNLOAD SEQUENCE IN FASTA
EARLY residues
21: Y;
22: F;
23: Y;
31: Q;
33: F;
35: Y;
45: F;
CLICK TO DOWNLOAD LIST OF RESIDUES