Entry STF0057
cellular retinol-binding protein type-2
Protein information
| Name of the protein: | Retinol-binding protein 2 |
| Organism: | Rattus norvegicus |
| Number of residues: | 134 |
| Related UniProt entry: | P06768 (Fragment: 1 - 135) |
| Related PDB entry: | 1OPA |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 131
Related publication:
PMID 19965581
Experiment details: "For the identification of slow exchanging amide protons, the protein sample buffer was replaced with a perdeuterated solution consisting of 20 mM KD2PO4 and 0.05% NaN3 in D2O, as previously described (36). This perdeuterated solution was prepared from the protonated buffer (90% H2O/10% D2O) at pH 6.0 by lyophilizing and redissolving in D2O twice. The buffer exchange was performed at 4°C with Vivaspin centrifugal concentrators (molecular weight cutoff of 10 kDa) in several rounds of filtration over 6–10 h. Following equilibration to 25°C inside the NMR magnet, a series of homonuclear TOCSY (alternating beween 30 and 80 ms spin lock time) and NOESY (alternating between 80 and 150 ms mixing time) experiments were collected over a period of more than 9 days (the first 3.5 days at 600.13 MHz and the remaining time at 499.87 MHz) in order to monitor the amide proton exchange."
Protection threshold: no exchange for >220 hours
Sequence:
MTKDQNGTWEMESNENFEGYMKALDIDFATRKIAVRLTQTKIIVQDGDNFKTKTNSTFRNYDLDFTVGVEFDEHTKGLDGRNVKTLVTWEGNTLVCVQKGEKENRGWKQWVEGDKLYLELTCGDQVCRQVFKKK
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STRONG residues
9: W;
10: E;
43: I;
44: V;
88: T;
94: L;
95: V;
98: Q;
107: W;
108: K;
109: Q;
110: W;
116: L;
117: Y;
118: L;
119: E;
120: L;
121: T;
127: C;
129: Q;
130: V;
132: K;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 131
Related publication:
PMID 19965581
Experiment details: "For the identification of slow exchanging amide protons, the protein sample buffer was replaced with a perdeuterated solution consisting of 20 mM KD2PO4 and 0.05% NaN3 in D2O, as previously described (36). This perdeuterated solution was prepared from the protonated buffer (90% H2O/10% D2O) at pH 6.0 by lyophilizing and redissolving in D2O twice. The buffer exchange was performed at 4°C with Vivaspin centrifugal concentrators (molecular weight cutoff of 10 kDa) in several rounds of filtration over 6–10 h. Following equilibration to 25°C inside the NMR magnet, a series of homonuclear TOCSY (alternating beween 30 and 80 ms spin lock time) and NOESY (alternating between 80 and 150 ms mixing time) experiments were collected over a period of more than 9 days (the first 3.5 days at 600.13 MHz and the remaining time at 499.87 MHz) in order to monitor the amide proton exchange."
Protection threshold: no exchange for >20 hours
Sequence:
MTKDQNGTWEMESNENFEGYMKALDIDFATRKIAVRLTQTKIIVQDGDNFKTKTNSTFRNYDLDFTVGVEFDEHTKGLDGRNVKTLVTWEGNTLVCVQKGEKENRGWKQWVEGDKLYLELTCGDQVCRQVFKKK
CLICK TO DOWNLOAD SEQUENCE IN FASTA
MEDIUM residues
22: K;
23: A;
24: L;
50: F;
51: K;
52: T;
71: F;
86: L;
87: V;
97: V;
122: C;
131: F;
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