Entry STF0056
cellular retinol-binding protein type-1
Protein information
Name of the protein: | Retinol-binding protein 1 |
Organism: | Rattus norvegicus |
Number of residues: | 134 |
Related UniProt entry: | P02696 (Fragment: 1 - 135) |
Related PDB entry: | 1JBH |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 124
Related publication:
PMID 19965581
Experiment details: "For the identification of slow exchanging amide protons, the protein sample buffer was replaced with a perdeuterated solution consisting of 20 mM KD2PO4 and 0.05% NaN3 in D2O, as previously described (36). This perdeuterated solution was prepared from the protonated buffer (90% H2O/10% D2O) at pH 6.0 by lyophilizing and redissolving in D2O twice. The buffer exchange was performed at 4°C with Vivaspin centrifugal concentrators (molecular weight cutoff of 10 kDa) in several rounds of filtration over 6–10 h. Following equilibration to 25°C inside the NMR magnet, a series of homonuclear TOCSY (alternating beween 30 and 80 ms spin lock time) and NOESY (alternating between 80 and 150 ms mixing time) experiments were collected over a period of more than 9 days (the first 3.5 days at 600.13 MHz and the remaining time at 499.87 MHz) in order to monitor the amide proton exchange."
Protection threshold: no exchange for >220 hours
Sequence:
MPVDFNGYWKMLSNENFEEYLRALDVNVALRKIANLLKPDKEIVQDGDHMIIRTLSTFRNYIMDFQVGKEFEEDLTGIDDRKCMTTVSWDGDKLQCVQKGEKEGRGWTQWIEGDELHLEMRAEGVTCKQVFKKVH
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STRONG residues
7: G;
9: W;
10: K;
12: L;
20: Y;
21: L;
22: R;
23: A;
24: L;
41: K;
42: E;
43: I;
44: V;
50: M;
51: I;
52: I;
53: R;
65: F;
71: F;
85: T;
88: S;
93: K;
95: Q;
96: C;
107: W;
108: T;
109: Q;
110: W;
112: E;
116: L;
117: H;
118: L;
119: E;
120: M;
121: R;
122: A;
125: V;
127: C;
129: Q;
130: V;
131: F;
132: K;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 124
Related publication:
PMID 19965581
Experiment details: "For the identification of slow exchanging amide protons, the protein sample buffer was replaced with a perdeuterated solution consisting of 20 mM KD2PO4 and 0.05% NaN3 in D2O, as previously described (36). This perdeuterated solution was prepared from the protonated buffer (90% H2O/10% D2O) at pH 6.0 by lyophilizing and redissolving in D2O twice. The buffer exchange was performed at 4°C with Vivaspin centrifugal concentrators (molecular weight cutoff of 10 kDa) in several rounds of filtration over 6–10 h. Following equilibration to 25°C inside the NMR magnet, a series of homonuclear TOCSY (alternating beween 30 and 80 ms spin lock time) and NOESY (alternating between 80 and 150 ms mixing time) experiments were collected over a period of more than 9 days (the first 3.5 days at 600.13 MHz and the remaining time at 499.87 MHz) in order to monitor the amide proton exchange."
Protection threshold: no exchange for >20 hours
Sequence:
MPVDFNGYWKMLSNENFEEYLRALDVNVALRKIANLLKPDKEIVQDGDHMIIRTLSTFRNYIMDFQVGKEFEEDLTGIDDRKCMTTVSWDGDKLQCVQKGEKEGRGWTQWIEGDELHLEMRAEGVTCKQVFKKVH
CLICK TO DOWNLOAD SEQUENCE IN FASTA
MEDIUM residues
13: S;
25: D;
40: D;
83: C;
86: T;
87: V;
97: V;
111: I;
115: E;
128: K;
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