Entry STF0054
E.coli YOJN histidine-phosphotransferase domain
Protein information
| Name of the protein: | Phosphotransferase RcsD |
| Organism: | Escherichia coli (strain K12) |
| Number of residues: | 116 |
| Related UniProt entry: | P39838 (Fragment: 775 - 890) |
| Related PDB entry: | 1SR2 |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 7.0; 20.0 Celsius; Probes: all amides
Related publication:
PMID 15476819
Experiment details: "The proton-exchange experiments were carried out on a Bruker DMX 600 spectrometer at 293 K. The 15N-labeled YojN-HPt sample was lyophilized and dissolved in the same volume of ice-cold D2O. A series of [1H,15N]-HSQC spectra were acquired every 30 minutes during the first two hours and thereafter every two hours for the following two days."
Protection threshold: residues that exhibit significant protection (no exchange for > 2 hours)
Sequence:
MQEAVLQLIEVQLAQEEVTESPLGGDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPGKQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL
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STRONG residues
48: V;
49: K;
50: R;
51: L;
52: Y;
55: A;
56: A;
63: L;
64: A;
65: Q;
66: T;
67: A;
68: H;
69: R;
70: L;
71: K;
72: G;
73: V;
74: F;
75: A;
76: M;
77: L;
78: N;
79: L;
82: G;
83: K;
85: L;
86: C;
87: E;
89: L;
90: E;
92: L;
93: I;
95: E;
96: K;
97: D;
98: V;
101: I;
102: E;
103: K;
104: Y;
105: I;
106: S;
107: D;
108: I;
109: D;
110: S;
111: Y;
112: V;
113: K;
114: S;
115: L;
116: L;
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