Entry STF0051
Oxidized P. aeruginosa ferricytochrome c(551)
Protein information
| Name of the protein: | Cytochrome c-551 |
| Organism: | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
| Number of residues: | 82 |
| Related UniProt entry: | P00099 (Fragment: 23 - 104) |
| Related PDB entry: | 451C |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 31
Related publication:
PMID 12459911
Experiment details: "Hydrogen-exchange rates were measured by monitoring the change in the TOCSY or 1D amide proton peak intensity as a function of time after exchanging a lyophilized sample into D2O. To initiate hydrogen exchange, a sample of oxidized Pa cyt c551 (3.0 mM protein, 5 times molar excess K3[Fe(CN)6], 50 mM NaPi pH 6.0) was lyophilized and dissolved in D2O. The pH* (uncorrected pH value) was immediately adjusted to 6.0 and data acquisition began 8 min after commencement of exchange. 1D (8 scans) and 2D TOCSY (32 scans) spectra were collected without solvent suppression using a Varian INOVA 500 MHz spectrometer at 300 K. Each TOCSY spectrum (4,096 ·256 points, with a 1.1-s recycle time) took about 5 h to acquire. TOCSY data were collected at 8, 13, 18, 25, 32, 37, 42, 96, and 317 h after initiation of exchange."
Protection threshold: log(P) >~ 5
Sequence:
EDPEVLFKNKGCVACHAIDTKMVGPAYKDVAAKFAGQAGAEAELAQRIKNGSQGVWGPIPMPPNAVSDDEAQTLAKWVLSQK
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STRONG residues
16: H;
47: R;
49: K;
50: N;
51: G;
52: S;
61: M;
72: Q;
74: L;
76: K;
77: W;
79: L;
80: S;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 31
Related publication:
PMID 12459911
Experiment details: "Hydrogen-exchange rates were measured by monitoring the change in the TOCSY or 1D amide proton peak intensity as a function of time after exchanging a lyophilized sample into D2O. To initiate hydrogen exchange, a sample of oxidized Pa cyt c551 (3.0 mM protein, 5 times molar excess K3[Fe(CN)6], 50 mM NaPi pH 6.0) was lyophilized and dissolved in D2O. The pH* (uncorrected pH value) was immediately adjusted to 6.0 and data acquisition began 8 min after commencement of exchange. 1D (8 scans) and 2D TOCSY (32 scans) spectra were collected without solvent suppression using a Varian INOVA 500 MHz spectrometer at 300 K. Each TOCSY spectrum (4,096 ·256 points, with a 1.1-s recycle time) took about 5 h to acquire. TOCSY data were collected at 8, 13, 18, 25, 32, 37, 42, 96, and 317 h after initiation of exchange."
Protection threshold: 4 < log(P) < 5
Sequence:
EDPEVLFKNKGCVACHAIDTKMVGPAYKDVAAKFAGQAGAEAELAQRIKNGSQGVWGPIPMPPNAVSDDEAQTLAKWVLSQK
CLICK TO DOWNLOAD SEQUENCE IN FASTA
MEDIUM residues
15: C;
17: A;
24: G;
28: K;
30: V;
31: A;
32: A;
33: K;
44: L;
45: A;
46: Q;
48: I;
55: V;
64: N;
71: A;
73: T;
75: A;
78: V;
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