Entry STF0042
Hen egg-white lysozyme
Protein information
Name of the protein: | Lysozyme C |
Organism: | Gallus gallus |
Number of residues: | 129 |
Related UniProt entry: | P00698 (Fragment: 19 - 147) |
Related PDB entry: | 1HEL |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 7.5; 30.0 Celsius; Probes: 68
Related publication:
PMID 1409571
Experiment details: "Exchange was carried out at 30°C in a D2O incubation mixture containing 0.1 M NaC1, 3 mM sodium azide, and 20 mM sodium phosphate buffer, pH 7.5. (uncorrected). Hydrogen exchange was initiated by dissolution of lyophilized protein in the exchange buffer to give a protein concentration of 2.5 mg/ml (0.17 mM). This protein concentration was chosen so that aggregation of lysozyme was negligible. Sixteen samples were withdrawn from the incubation mixture after time intervals ranging from 40 sec to 70 days and the exchange quenched by addition of 2.3 ml of 240 mM deuterated sodium acetate buffer, pH 3.6, giving a final pH of 3.8."
Protection threshold: very slowly exchanging amide protons; k(ex) < E-07
Sequence:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
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STRONG residues
12: M;
28: W;
29: V;
30: C;
31: A;
58: I;
95: A;
96: K;
98: I;
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EARLY
Method: HDX-folding competition by NMR
Conditions: pH 4.5-9.5; None Celsius; Probes: 65
Related publication:
PMID 2000138
Experiment details: "Refolding experiments were carried out between pH values of 4.5 and 9.5."
Protection threshold: high degree of protection relative to exchange (% exchange < 30)
Sequence:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
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EARLY residues
8: L;
9: A;
10: A;
11: A;
12: M;
13: K;
15: H;
17: L;
23: Y;
27: N;
28: W;
29: V;
30: C;
31: A;
32: A;
33: K;
34: F;
35: E;
63: W;
64: C;
78: I;
92: V;
93: N;
94: C;
95: A;
96: K;
97: K;
98: I;
99: V;
108: W;
111: W;
112: R;
115: C;
123: W;
124: I;
125: R;
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EARLY
Method: Pulse labeling HDX NMR
Conditions: pH 5.5; 20.0 Celsius; Probes: 48
Related publication:
PMID 1641003
Experiment details: "Experiments were done at 20 °C using a Biologic QFM5 rapid mixing quench flow apparatus. Lysozyme (20 mg/ml) was initially dissolved in 6M GdnDCl in D2O at pH 6, leading to complete denaturation and substitution of all exchangeable hydrogens by deuterium. Refolding was initiated by dilution of this solution 10-fold into 20 mM sodium acetate, pH 5.5 in H2O. At the resulting pH of 5.2 the half life for amide exchange is about 16 s, so that negligible labelling occurred during this phase. After variable refolding times (3.5-2000 ms) the solution was diluted again with a volume five times that of the initial protein solution of 0.2 M sodium borate, pH 10.0. This initiated labelling at a pH of 9.5. After 8.4 ms the labelling pulse was terminated by further dilution, with a volume again five times that of the initial volume of protein solution, of 0.5 M acetic acid in H2O. The final pH was about 4, at which exchange in the native protein of the investigated amides is very slow."
Protection threshold: refolding time constant < 3 ms
Sequence:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
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EARLY residues
12: M;
29: V;
63: W;
78: I;
96: K;
99: V;
124: I;
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INTERMEDIATE
Method: Pulse labeling HDX NMR
Conditions: pH 5.5; 20.0 Celsius; Probes: 48
Related publication:
PMID 1641003
Experiment details: "Experiments were done at 20 °C using a Biologic QFM5 rapid mixing quench flow apparatus. Lysozyme (20 mg/ml) was initially dissolved in 6M GdnDCl in D2O at pH 6, leading to complete denaturation and substitution of all exchangeable hydrogens by deuterium. Refolding was initiated by dilution of this solution 10-fold into 20 mM sodium acetate, pH 5.5 in H2O. At the resulting pH of 5.2 the half life for amide exchange is about 16 s, so that negligible labelling occurred during this phase. After variable refolding times (3.5-2000 ms) the solution was diluted again with a volume five times that of the initial protein solution of 0.2 M sodium borate, pH 10.0. This initiated labelling at a pH of 9.5. After 8.4 ms the labelling pulse was terminated by further dilution, with a volume again five times that of the initial volume of protein solution, of 0.5 M acetic acid in H2O. The final pH was about 4, at which exchange in the native protein of the investigated amides is very slow."
Protection threshold: 3 ms < refolding time constant < 6 ms
Sequence:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
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INTERMEDIATE residues
8: L;
10: A;
11: A;
28: W;
31: A;
64: C;
92: V;
94: C;
95: A;
108: W;
111: W;
112: R;
123: W;
125: R;
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