Entry STF0041
Bacillus amyloliquefaciens ribonuclease
Protein information
Name of the protein: | Ribonuclease |
Organism: | Bacillus amyloliquefaciens |
Number of residues: | 110 |
Related UniProt entry: | P00648 (Fragment: 48 - 157) |
Related PDB entry: | 1A2P |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.5-7.6; 33.0 Celsius; Probes: 41
Related publication:
PMID 7626599
Experiment details: "20 mg of lyophilized l5N-labeled protein was in the appropriate buffer containing 90% H2O and 10% D2O. The exchange buffer was 20 mM deuterated imidazole, pD 6.7, or 50 mM deuterated Tris, pD 7.9, containing 0.05% sodium azide, dissolved in D2O. Approximately 20 mg of lyophilized, uniformly l5N-labeled protein was dissolved in the exchange buffer (final concentration ~3 mM), centrifuged, transferred to an NMR tube, and allowed to equilibrate in the magnet for 10 min before the start of the first NMR experiment. The first spectrum was recorded approximately 20 min after the solution of the protein. The sample was kept at 33 or 37 °C, in the NMR tubes, throughout the study, which lasted up to 3 months."
Protection threshold: k(obs) < E-05/min at pH 6.5 and k(obs) < 2E-04/min at pH 7.6
Sequence:
AQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYQTFTKIR
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STRONG residues
14: L;
25: I;
74: A;
89: L;
97: Y;
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