Native exchange NMR None ΔG(HX) > 7 kcal/mol GSPEFLGEEDIPREPRRIVIHRGSTGLGFNIIGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEANSRVNSSGRIVTN

The experiment was done in D2O using 15N-labeled PDZ-3 at pDread 6.0 and 25 °C. The well-dispersed cross-peaks allow the exchange rate constants, k(ex), to be measured accurately for slowly exchanging amide protons.

Native exchange NMR None 5 < ΔG(HX) (kcal/mol) < 7 GSPEFLGEEDIPREPRRIVIHRGSTGLGFNIIGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEANSRVNSSGRIVTN

The experiment was done in D2O using 15N-labeled PDZ-3 at pDread 6.0 and 25 °C. The well-dispersed cross-peaks allow the exchange rate constants, k(ex), to be measured accurately for slowly exchanging amide protons.

Native exchange NMR None ΔGHX < 5 kcal/mol GSPEFLGEEDIPREPRRIVIHRGSTGLGFNIIGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEANSRVNSSGRIVTN

The experiment was done in D2O using 15N-labeled PDZ-3 at pDread 6.0 and 25 °C. The well-dispersed cross-peaks allow the exchange rate constants, k(ex), to be measured accurately for slowly exchanging amide protons.