Entry STF0034
Borrelia burgdorferi outer surface protein A (OspA)
Protein information
| Name of the protein: | Outer surface protein A |
| Organism: | Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31) |
| Number of residues: | 257 |
| Related UniProt entry: | P0CL66 (Fragment: 18 - 273) |
| Related PDB entry: | 1OSP |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.0; 37.0 Celsius; Probes: 150
Related publication:
PMID 12381326
Experiment details: "15N- and 13C, 15N-labeled OspA was prepared as described. HX was initiated by rapid buffer exchange of 15N-labeled, 1 mM OspA using a Sephadex G25 (Pharmcia) spin column to D2O buffer containing 10 mM sodium phosphate and 0 – 0.6 M Gu2HCl. This buffer contained NaCl, so that the sum of [Gu2HCl] and [NaCl] was kept at 0.65 M. Immediately following buffer exchange, a series of 1H, 15N-HSQC spectra were recorded at 37 °C on a Varian 600 spectrometer. The pH (uncorrected) of each sample was determined after NMR measurements."
Protection threshold: ΔG(HX0) > 10; m-value > 10
Sequence:
KQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSGVLEGVKADKSKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEITKLDEIKNALK
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STRONG residues
1: K;
2: Q;
3: N;
4: V;
5: S;
6: S;
7: L;
8: D;
9: E;
10: K;
11: N;
12: S;
13: V;
14: S;
15: V;
16: D;
17: L;
18: P;
19: G;
20: E;
21: M;
22: K;
23: V;
24: L;
25: V;
26: S;
27: K;
28: E;
29: K;
30: N;
31: K;
32: D;
33: G;
34: K;
35: Y;
36: D;
37: L;
38: I;
39: A;
40: T;
41: V;
42: D;
43: K;
44: L;
45: E;
46: L;
47: K;
48: G;
49: T;
50: S;
51: D;
52: K;
53: N;
54: N;
55: G;
56: S;
57: G;
58: V;
59: L;
60: E;
61: G;
62: V;
63: K;
64: A;
65: D;
66: K;
67: S;
68: K;
69: V;
70: K;
71: L;
72: T;
73: I;
74: S;
75: D;
76: D;
77: L;
78: G;
79: Q;
80: T;
81: T;
82: L;
83: E;
84: V;
85: F;
86: K;
87: E;
88: D;
89: G;
90: K;
91: T;
92: L;
93: V;
94: S;
95: K;
96: K;
97: V;
98: T;
99: S;
100: K;
101: D;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 6.0; 37.0 Celsius; Probes: 150
Related publication:
PMID 12381326
Experiment details: "15N- and 13C, 15N-labeled OspA was prepared as described. HX was initiated by rapid buffer exchange of 15N-labeled, 1 mM OspA using a Sephadex G25 (Pharmcia) spin column to D2O buffer containing 10 mM sodium phosphate and 0 – 0.6 M Gu2HCl. This buffer contained NaCl, so that the sum of [Gu2HCl] and [NaCl] was kept at 0.65 M. Immediately following buffer exchange, a series of 1H, 15N-HSQC spectra were recorded at 37 °C on a Varian 600 spectrometer. The pH (uncorrected) of each sample was determined after NMR measurements."
Protection threshold: 7 < ΔG(HX0) < 10; m-value > 7
Sequence:
KQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSGVLEGVKADKSKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEITKLDEIKNALK
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MEDIUM residues
102: K;
103: S;
104: S;
105: T;
106: E;
107: E;
108: K;
109: F;
110: N;
111: E;
112: K;
113: G;
114: E;
115: V;
116: S;
117: E;
118: K;
119: I;
120: I;
121: T;
122: R;
123: A;
124: D;
125: G;
126: T;
127: R;
128: L;
129: E;
130: Y;
131: T;
132: G;
133: I;
134: K;
135: S;
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WEAK
Method: Native exchange NMR
Conditions: pH 6.0; 37.0 Celsius; Probes: 150
Related publication:
PMID 12381326
Experiment details: "15N- and 13C, 15N-labeled OspA was prepared as described. HX was initiated by rapid buffer exchange of 15N-labeled, 1 mM OspA using a Sephadex G25 (Pharmcia) spin column to D2O buffer containing 10 mM sodium phosphate and 0 – 0.6 M Gu2HCl. This buffer contained NaCl, so that the sum of [Gu2HCl] and [NaCl] was kept at 0.65 M. Immediately following buffer exchange, a series of 1H, 15N-HSQC spectra were recorded at 37 °C on a Varian 600 spectrometer. The pH (uncorrected) of each sample was determined after NMR measurements."
Protection threshold: 5 < ΔG(HX0) < 8; 5 < m-value < 6
Sequence:
KQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSGVLEGVKADKSKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEITKLDEIKNALK
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WEAK residues
157: E;
158: K;
159: T;
160: T;
161: L;
162: V;
163: V;
164: K;
165: E;
166: G;
167: T;
168: V;
169: T;
170: L;
171: S;
172: K;
173: N;
174: I;
175: S;
176: K;
177: S;
178: G;
179: E;
180: V;
181: S;
182: V;
183: E;
184: L;
185: N;
186: D;
187: T;
188: D;
189: S;
190: S;
191: A;
192: A;
193: T;
194: K;
195: K;
196: T;
197: A;
198: A;
199: W;
200: N;
201: S;
202: G;
203: T;
204: S;
205: T;
206: L;
207: T;
208: I;
209: T;
210: V;
211: N;
212: S;
213: K;
214: K;
215: T;
216: K;
217: D;
218: L;
219: V;
220: F;
221: T;
222: K;
223: E;
224: N;
225: T;
226: I;
227: T;
228: V;
229: Q;
230: Q;
231: Y;
232: D;
233: S;
234: N;
235: G;
236: T;
237: K;
238: L;
239: E;
240: G;
241: S;
242: A;
243: V;
244: E;
245: I;
246: T;
247: K;
248: L;
249: D;
250: E;
251: I;
252: K;
253: N;
254: A;
255: L;
256: K;
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