Entry STF0030
Apo-flavodoxin
Protein information
| Name of the protein: | Flavodoxin-2 |
| Organism: | Azotobacter vinelandii |
| Number of residues: | 179 |
| Related UniProt entry: | P00324 (Fragment: 2 - 180) |
| Related PDB entry: | 1YOB |
Visualize the data
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Experiment sets
EARLY
Method: Denaturant-dependent disappearance of amide cross peaks by NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 114
Related publication:
PMID 19170491
Experiment details: "Unfolded apoflavodoxin was obtained by denaturing flavodoxin in 6 M guanidine hydrochloride. Subsequently, FMN was removed via gel filtration at 7 M GuHCl. 550 μL NMR samples of apoflavodoxin at various denaturant concentrations were prepared; in total, 18 samples with GuHCl concentrations ranging from 4.05 to 1.58 M GuHCl. The buffer used in all experiments was 100 mM potassium pyrophosphate, pH 6.0. 1H-15N HSQC spectra of unfolded apoflavodoxin were acquired. Subsequently, the chemical shifts of the amides of the unfolded protein were determined in these spectra. In addition, the denaturant-dependent disappearance of the amide cross peaks of unfolded apoflavodoxin were tracked."
Protection threshold: 2.5 M GuHCl < Cm (midpoint of folding) < 2.8 M GuHCl
Sequence:
AKIGLFFGSNTGKTRKVAKSIKKRFDDETMSDALNVNRVSAEDFAQYQFLILGTPTLGEGELPGLSSDAENESWEEFLPKIEGLDFSGKTVALFGLGDQVGYPENYLDALGELYSFFKDRGAKIVGSWSTDGYEFESSEAVVDGKFVGLALDLDNQSGKTDERVAAWLAQIAPEFGLSL
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EARLY residues
52: L;
53: G;
78: L;
82: E;
87: S;
92: A;
109: A;
111: G;
115: S;
126: G;
127: S;
148: G;
165: A;
168: L;
169: A;
170: Q;
171: I;
172: A;
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INTERMEDIATE
Method: Denaturant-dependent disappearance of amide cross peaks by NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 114
Related publication:
PMID 19170491
Experiment details: "Unfolded apoflavodoxin was obtained by denaturing flavodoxin in 6 M guanidine hydrochloride. Subsequently, FMN was removed via gel filtration at 7 M GuHCl. 550 μL NMR samples of apoflavodoxin at various denaturant concentrations were prepared; in total, 18 samples with GuHCl concentrations ranging from 4.05 to 1.58 M GuHCl. The buffer used in all experiments was 100 mM potassium pyrophosphate, pH 6.0. 1H-15N HSQC spectra of unfolded apoflavodoxin were acquired. Subsequently, the chemical shifts of the amides of the unfolded protein were determined in these spectra. In addition, the denaturant-dependent disappearance of the amide cross peaks of unfolded apoflavodoxin were tracked."
Protection threshold: 2.2 M GuHCl < Cm (midpoint of folding) < 2.5 M GuHCl
Sequence:
AKIGLFFGSNTGKTRKVAKSIKKRFDDETMSDALNVNRVSAEDFAQYQFLILGTPTLGEGELPGLSSDAENESWEEFLPKIEGLDFSGKTVALFGLGDQVGYPENYLDALGELYSFFKDRGAKIVGSWSTDGYEFESSEAVVDGKFVGLALDLDNQSGKTDERVAAWLAQIAPEFGLSL
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INTERMEDIATE residues
46: Q;
54: T;
83: G;
86: F;
95: G;
101: G;
122: A;
129: S;
130: T;
143: D;
144: G;
150: A;
167: W;
176: G;
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STRONG
Method: Native exchange NMR
Conditions: pH 6.2; 30.0 Celsius; Probes: 57+2
Related publication:
PMID 9737928
Experiment details: "The H/D exchange measurements were carried out on the resulting 2 mM 15N-labeled apoflavodoxin sample over a five-day period (pH* 6.2, 303 K). The first gradient-enhanced 1H-15N HSQC experiment was started 12 minutes after initiation of exchange and each experiment lasted eight minutes. Peak intensities were measured and used to calculate the amide proton exchange rate, k(ex). ΔG(op) values were determined from the observed rate constants, k(ex), and the intrinsic chemical exchange rates, k(int)."
Protection threshold: ΔG(op) ≥ 6.2 kcal/mol
Sequence:
AKIGLFFGSNTGKTRKVAKSIKKRFDDETMSDALNVNRVSAEDFAQYQFLILGTPTLGEGELPGLSSDAENESWEEFLPKIEGLDFSGKTVALFGLGDQVGYPENYLDALGELYSFFKDRGAKIVGSWSTDGYEFESSEAVVDGKFVGLALDLDNQSGKTDERVAAWLAQIAPEFGLSL
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STRONG residues
5: L;
6: F;
49: F;
50: L;
51: I;
52: L;
53: G;
81: I;
90: T;
91: V;
92: A;
93: L;
94: F;
111: G;
114: Y;
117: F;
118: K;
120: R;
123: K;
125: V;
165: A;
167: W;
168: L;
169: A;
170: Q;
171: I;
172: A;
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