Start2Fold

The database of hydrogen/deuterium exchange data on protein folding and stability

Entry STF0029

Barstar

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Protein information

Name of the protein: Barstar
Organism: Bacillus amyloliquefaciens
Number of residues: 89
Related UniProt entry:   P11540 (Fragment: 2 - 90)
Related PDB entry:   1BTA

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Experiment sets

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STRONG

Method: Native exchange NMR

Conditions: pH 6.7; 32.0 Celsius; Probes: 13 +2 Trp indole protons

Related publication:
 PMID 9517545

Experiment details: "The protection factors were defined based on exchange measurements with different GdnHCl concentrations. D2O and H2O buffer solutions contained 20 mM sodium phosphate at pH 6.7, 32 °C. The reported pH of the D2O buffer is the uncorrected pH meter reading. Protection factors are provided for 0.33 M GdnHCl."

Protection threshold: P > 6000

Sequence: KKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTGWVEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKAEGCDITIILS
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STRONG residues

8: E; 11: R; 49: L; 72: Q; 78: K; 87: I;
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MEDIUM

Method: Native exchange NMR

Conditions: pH 6.7; 32.0 Celsius; Probes: 13 +2 Trp indole protons

Related publication:
 PMID 9517545

Experiment details: "The protection factors were defined based on exchange measurements with different GdnHCl concentrations. D2O and H2O buffer solutions contained 20 mM sodium phosphate at pH 6.7, 32 °C. The reported pH of the D2O buffer is the uncorrected pH meter reading. Protection factors are provided for 0.33 M GdnHCl."

Protection threshold: 2000 < P < 6000

Sequence: KKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTGWVEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKAEGCDITIILS
 CLICK TO DOWNLOAD SEQUENCE IN FASTA

MEDIUM residues

16: L; 23: E; 34: L; 38: W; 39: D; 44: W; 45: V; 50: V; 52: E;
 CLICK TO DOWNLOAD LIST OF RESIDUES